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Astrocytes incorporate neurocan into a pericellular, substrate-bound ECM in vitro. Living astrocytes were labeled with the anti-neurocan mAb 1G2 (b), fixed in methanol, and labeled with rabbit antibodies against GFAP (a). Labeling for neurocan was seen on the substrate around GFAP-positive cells. Scale bar, 25 μm.
