Fig. 8.

Cytokines influence neurocan expression in cultured astrocytes. The cells were grown for 6 d in the presence of 10 ng/ml of the cytokine, and the conditioned medium was concentrated and digested with chondroitinase ABC and an equal volume (65 μl) was applied to each lane. The blot was labeled with the anti-neurocan mAb 1G2. An increase in the amount of neurocan was seen in response to TGFβ and to a greater extent with EGF. PDGF, IFN-γ, and IL-1β brought about a decline in the amount of neurocan that was detected.