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Cerebral cortical neurons in newborn FGF2−/− mice. Shown is NeuN immunostaining in the frontal cortex of wild-type (a) and FGF2−/− mice (b) at P1. The bar at left indicates the boundary between supragranular and infragranular layers. Note the prominent nuclear staining of large, pyramidal-shaped cells in layer 5/6 (arrows) in wild-type mice that is absent in FGF2−/− mice. No difference in neuronal number is apparent in upper layers. Pial side is at the top. Scale bar, 100 μm.
