Fig. 2.

Effects of sense or antisense plasmid administration on 5-HTT activity and levels. [³H]5-HT synaptosomal uptake was measured in the hypothalamus (HYP), the hippocampus (HI), the striatum (ST), and the anterior cortex (ANT CX), 3 d (top) or 7 d (bottom) after intra-DRN injection of sense or antisense construct, respectively. Membrane binding assays were performed with 0.7 nm[³H]citalopram in the same brain regions and in the anterior raphe area (RA). Variations in both parameters are expressed as percentage of control values (rats injected with the nonrecombinant pRc-CMV and killed 3 or 7 d later). Data are the means ± SEM of values in three independent experiments, each performed with five rats. Control values for [³H]5-HT uptake, in femtomoles per minute per milligram of protein are: 255 ± 11 (HYP), 136 ± 9 (HI), 210 ± 7 (ST), and 227 ± 8 (ANT CX). Control values for [³H]citalopram specific binding, in femtomoles per milligram of protein are: 228 ± 21 (RA), 365 ± 20 (HYP), 155 ± 9 (HI), 264 ± 14 (ST), and 226 ± 21 (ANT CX). *p < 0.05; **p < 0.01; ***p < 0.005 as compared with paired-control rats (Student's ttest).