Fig. 3.
Developmental changes in flip–flop splice variants indicated by changes in effectiveness of cyclothiazide.A, Long pulses (2 sec) of 10 mm glutamate in the continued presence of 60 μm CTZ show marked desensitization in E11 (gray trace) and E18 (black trace) patches. Traces scaled to same peak value. Double-exponential fits to the traces with the following parameters are shown: E11, τfast 86 msec, τslow 588 msec, 28% fast phase, 27% steady-state current; E18, τfast 51 msec, τslow 462 msec, 38% fast phase, 7% steady-state current. B, The current remaining after desensitization, measured as steady-state/peak ratio in which steady-state current was obtained from best single- or double-exponential fits to the traces during onset of desensitization. n = 5 and 11 patches for E11 and E18, respectively. *p < 0.05 indicates significant difference.