Fig. 6.

Translation profile of MBP mRNAs in the qk^v/wt and qk^v/qk^v littermate brainstem. Cytoplasmic extract was prepared from one P20 brainstem derived from each littermate and fractionated through parallel linear sucrose gradients (15–45% w/w) to separate polyribosomes and nontranslating ribosomal components, as depicted on top of the corresponding lanes. In addition, EDTA was used to dissociate polyribosomes into ribosomal subunits and to release the mRNAs. Twelve fractions were obtained from each gradient followed by RPA analysis.