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. 2000 Jul 15;20(14):5292–5299. doi: 10.1523/JNEUROSCI.20-14-05292.2000

Fig. 7.

Fig. 7.

IL-1β induction of NF-κB binding is downregulated by breakdown of extracellular nucleotides by apyrase. Astrocyte cultures were treated with IL-1β in the presence or absence of apyrase (lane apy; 20 U/ml) pretreatment for 30 min or oATP (lane oATP) pretreatment as described previously. Nuclei were harvested at 30 min and subjected to EMSA with a radiolabeled oligonucleotide probe containing the NF-κB binding site along with wild-type (lane NF) and mutant (lane mut) competitor oligonucleotides. Control samples, IL-1β-treated samples, and IL-1β-treated samples after apyrase pretreatment or oATP are shown. Three shift complexes were observed (A–C) in control cells. Complex A was strongly induced by IL-1β and downregulated by oATP pretreatment, as well as by apyrase. Quantitative analysis of these data is shownbelow each lane and represents the densitometric ratios for complex A compared with the value for the complex A in the control sample (left lane). Data shown are representative of two separate experiments with cells from two different brains.