Table 1.
IL-1β induction of NF-κB transcriptional activation is downregulated by oATP1-a
| Brain# | Control | IL-1β1-b | oATP + IL-1β1-c | Reduction1-d |
|---|---|---|---|---|
| 1 | 61 ± 6 | 355 ± 9 | 216 ± 11 | 48% |
| 2 | 4 ± 1 | 51 ± 1 | 17 ± 2 | 72% |
| 3 | 19 ± 3 | 187 ± 25 | 65 ± 9 | 65% |
| 4 | 69 ± 7 | 204 ± 10.5 | 139 ± 9 | 49% |
| 5 | 52 ± 14 | 294 ± 24 | 151 ± 7 | 59% |
F1-a
Astrocytes were transfected with a luciferase reporter construct for NF-κB. Cells were harvested at 6 hr after cytokine treatment. Data are expressed as 103RLU. Statistical analysis by Wilcoxon matched paired test demonstrated that inhibition of NF-κB transcriptional activation by oATP was statistically significant (p > 0.03) over five separate experiments.
F1-b
Cells were treated with 10 ng/ml IL-1β.
F1-c
Cells were pretreated with 300 μm oATP.
F1-d
Calculated as described in Materials and Methods.