Fig. 2.

Whole-mount preparations of cerebellar cultures reacted at 15 DIV with antibody to nonphosphorylated neurofilament protein and processed by the peroxidase-antiperoxidase method (375× magnification). Purkinje cell recurrent axon collaterals are indicated by arrows. A, Purkinje cells from a culture continuously exposed to activity-blocking agents.B, Purkinje cells from an activity-blocked culture treated since explantation with BDNF, 25 ng/ml nutrient medium. Axonal sprouting was not evident in neurotrophin-treated activity-blocked cultures.