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. 2000 Jul 15;20(14):5329–5338. doi: 10.1523/JNEUROSCI.20-14-05329.2000

Fig. 6.

Fig. 6.

Function of ROCK in the maintenance of dendritic branches. A–E, Representative images of neurons 2 d after transfection and 100 μm Y-27632 treatment are shown. Neurons were transfected with mCD8 alone (A, B) or cotransfected with RhoAV14 (C, D) or ROCKΔ3 (E). E, Inset, The myc immunostaining for ROCKΔ3 is shown. Additionally, neurons inB and D were treated with 100 μm Y-27632 at the time of transfection (composite confocal images using 16× objective). F, Quantification of basal dendritic branch segment numbers of mCD8- and of mCD8 plus RhoAV14-expressing neurons with or without Y-27632 treatment is shown. Y-27632 treatment alone does not affect dendritic segment number (p = 0.31; control treatment,n = 23; Y-27632 treatment, n = 23). Y-27632 treatment blocks RhoAV14-associated dendritic segment reduction (***p < 0.001; RhoAV14 + control,n = 17; RhoAV14 expression + Y-27632 treatment,n = 24). G, Y-27632 application does not alter the basal dendritic spine density of neurons expressing mCD8 alone (p = 0.65; n = 29, 21 for control, Y-27632 treatment, respectively). Y-27632 treatment is capable of restoring the spine density of neurons expressing RhoAV14 close to control level (p = 0.08;n = 16 for Y-27632 treatment and RhoA V14 expression). H, Activated ROCKΔ3 expression results in significant reduction of dendritic segments compared with that in mCD8 alone (p < 0.001 for both apical and basal;n = 19, 20, respectively, for ROCKΔ3). Scale bars, 50 μm.