Fig. 2.

Perikaryal inclusions in the absence of NF-L (A–D). Spinal cord sections stained with toluidine blue show the presence of hyaline inclusions in the perikarya of motor neurons (black arrowheads) from NF-L−/− (B), hNF-H transgenic (C), and hH;L−/− (D) mice at 2 years of age. Such inclusions were absent in motor neurons of normal (wt) mice (A). E, Immunohistochemistry revealing the presence of hNF-H (OC-95) and phosphorylated NF-H epitopes (SMI-31) in inclusions of both hH and hH;L−/− mice. The scale bar in D applies toA–D. Scale bars, 50 μm.