Fig. 2.

Dose–response relationship between concentration of APV in the culture medium and differentiation of Purkinje cells. Cerebellar neurons were cultured in the presence of various concentrations of APV. At 14 DIV, Purkinje cells were immunostained for calbindin D-28K. Images of Purkinje cells were obtained using a CCD camera. A shows representative example of Purkinje cells cultured with various concentrations of APV. B andC show the relationship between concentration of APV and maximum dendrite length or the number of branch points per longest dendrite in Purkinje cells, respectively. Data are means of experiments performed in triplicate; >30 neurons for each concentration were analyzed. Error bars indicate SEM. Scale bar, 50 μm.