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Pulse/chase analysis of proopiomelanocortin processing in wild-type AtT-20 cells (wt) and in AtT-20 cells overexpressing proSAAS (SAAS). Cells were labeled with 35S-Met for 10 min and then chased for 30 or 90 min in unlabeled Met, and then the media were immunoprecipitated with antisera to ACTH (left) or β-endorphin (right).Top, Exposure of the gel to x-ray film for 14 hr.Bottom, Exposure for 4 d.
