Fig. 3.

RAP does not interact directly with tPA.A, RAP (1 μg) and tPA (1 μg) were incubated in ACSF for 1 hr at 30°C. The incubation mixture was then divided and incubated with normal rabbit IgG (NR), anti-tPA IgG, or anti-RAP IgG, followed with immunoprecipitation (IP) without detergent. The immunoprecipitated materials were then immunoblotted (IB) with either anti-tPA IgG or anti-RAP IgG. B, RAP-Sepharose was incubated with¹²⁵I-tPA (5 nm) in ACSF for 1 hr at 30°C. After washing, both supernatant (S) and pellet (P) were analyzed via SDS-PAGE.