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. 2000 Jun 15;20(12):4535–4544. doi: 10.1523/JNEUROSCI.20-12-04535.2000

Fig. 4.

Fig. 4.

The extent of serine-14-phosphorylation on syn1A/B increases during the development of the rat brain. Rat brains of different ages (E, embryonic; P, postnatal; A, adult) were homogenized, and the postnuclear fraction was extracted with Triton X-100. Total protein (25 μg) from each lysate was resolved by SDS-PAGE, transferred to nitrocellulose, and probed with specific antibodies to detect phosphorylated syntaxin1A/B, syn1A/B, SNAP25, VAMP2, synaptotagmin, and nsec1. A, Representative Western blot results.B, Quantitative analysis of the relative amounts of detected proteins. The data for each protein were normalized to 100 arbitrary units at E18.