Skip to main content
. 2000 Jun 15;20(12):4596–4605. doi: 10.1523/JNEUROSCI.20-12-04596.2000

Fig. 1.

Fig. 1.

Coexpression of Sim, Fish, and Dfr in embryonic CNS midline cells detected via double-label immunostaining of P[3.7sim-lacZ] (A, B) and wild-type (C) embryos and confocal microscopy. A, A stage 9 P[3.7sim-lacZ] embryo immunostained with anti-β-gal (red) and anti-Fish (green) sera. Note strong overlapping expression (yellow) in the CNS midline cells. Fish is also strongly expressed in the lateral and cephalic neuroectoderm. B, A stage 10 P[3.7sim-lacZ] embryo immunostained with anti-β-gal (red) and anti-Dfr (green) sera. Note strong overlapping expression (yellow) in the CNS midline cells. Dfr is also strongly expressed in developing tracheal cells. C, A wild-type stage 11 embryo immunostained with anti-Fish (red) and anti-Dfr (green) sera. Note overlapping expression (yellow) in the CNS midline cells. Fish expression is not detected in tracheal cells. All views are ventral with anterior to left.