Fig. 1.

Effects of protein kinase activators on GluR1 phosphorylation in neostriatal slices. Neostriatal slices were prepared from normal C57BL/6 mice and incubated for 5 min in the absence (Control) or presence of the adenylyl cyclase activator forskolin (A) or the PKC activator PDBu (B). Levels of phospho-Ser⁸⁴⁵(A) or phospho-Ser⁸³¹(B) were determined by SDS-PAGE and immunoblotting. Phospho-GluR1 levels were detected, quantitated by densitometry, and expressed as percentage of the level detected in control slices. Arrows (top panels) indicate the position of the phospho-GluR1 bands in representative autoradiograms. Data (bottom panels) are expressed as means ± SEM for three experiments (*p < 0.05 compared with control, Mann–WhitneyU test).