Fig. 4.

Actin-binding proteins of dendritic spines were dispersed by actin depolymerization but unaffected by depolymerization of microtubules or detergent extraction. A,E, The actin-binding proteins α-actinin-2 (A) and drebrin (E) were found to be abundant within the dendritic spines of hippocampal neurons. B, F, After actin depolymerization with latrunculin A, the clusters dissociated, and both α-actinin-2 (B) and drebrin (F) became diffusely localized within the dendrites. C, D, G,H, Neither microtubule depolymerization with vincristine (C, G) nor detergent extraction (D, H) disrupted the clusters of α-actinin-2 (C, D) or drebrin (G, H). Scale bar, 10 μm.