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. 2000 Jun 15;20(12):4545–4554. doi: 10.1523/JNEUROSCI.20-12-04545.2000

Fig. 7.

Fig. 7.

Treatment with latrunculin A and detergent extraction affected synaptic clustering of different components of the PSD. A, Double immunostaining with CaMKIIα (red) and NR2A (green) showed that both proteins are concentrated within the PSDs of control neurons.B, After treatment with latrunculin A to depolymerize actin, CaMKIIα clusters dispersed (red), whereas the NR2A clusters remained intact (green).C, Similarly, clusters of GluR1 (red) and PSD-95 (green) were seen within control dendritic spines. D, After detergent extraction, GluR1 clusters were extracted (red), but PSD-95 clusters remained (green). E, F, After treatment with vincristine, neither PSD-95 (E, red) nor gephyrin (F, red) colocalizes with tubulin paracrystals (E, F, green). Scale bar, 10 μm.