Fig. 3.
An inhibitor of rod production is produced by E16 cells, and it signals through a LIFRβ/gp130 complex. P0 retinae were dissociated and cast at a density of 8 × 105cells/40 ml collagen gel. These gels were placed in the center of a culture well in a defined medium with or without the LIFRβ antagonist hLIF-05 (5 μg/ml). The gels were cultured in the presence of a surrounding collagen gel containing E16 or P0 cells at 4 × 106 cells/200 ml for 10 DIV. No contact between cells in the two gels was detected. The cells were then removed from the center gel of P0 cells and processed for immunocytochemistry, and the percentage of rhodopsin-positive cells was determined. Values represent the mean ± SD of three trials. For each trial, >300 cells were scored.