Table 1.
Quantification of antigen expression by heavily labeled cells after 15 DIV
| Cell type | Antibody | Reaggregate type | ||
|---|---|---|---|---|
| E16 | P0 | P0–E16 | ||
| Amacrine | mGluR2 | 25.7 ± 2.3 | 8.6 ± 2.0 | 12.4 ± 2.2 |
| Horizontal | Calbindin | 6.5 ± 1.4 | 0.01-a | 0.01-a |
| Cone | Cone opsins | 25.3 ± 3.8 | 0.01-a | 0.2 ± 0.3 |
| Rod | Rhodopsin | 20.0 ± 1.7 | 66.9 ± 4.9 | 14.7 ± 2.8 |
| Bipolar | 115A10 | 0.4 ± 0.8 | 7.6 ± 2.2 | 26.5 ± 5.2 |
| Müller | CRALBP | 0.01-a | 3.3 ± 1.3 | 4.2 ± 1.4 |
| Unidentified | 22.1 | 13.6 | 42.0 | |
Reaggregate cultures of E16, P0, or P0–E16 (1:20) were dissociated after 15 DIV. After they were processed for immunocytochemistry and autoradiography, birth-dated cells were scored for their labeling by the indicated antibodies. Values represent the mean ± SD of three to six trials. For each trial, ≥100 heavily labeled cells were assayed.
F1-a
Zero out of 300 heavily labeled cells were marker-positive.