Table 1.
Failure of Gαi subunits to specifically downregulate VMATs in BON cells
| [3H]Serotonin uptake (pmol/mg protein) | |
|---|---|
| Control | 1.58 ± 0.06 |
| Reserpine | 0.55 ± 0.01 |
| Gαi110 nm | 1.06 ± 0.02 |
| Gαi1 heat denatured | 1.11 ± 0.06 |
| Gαi2 10 nm | 1.04 ± 0.1 |
| Gαi2 heat denatured | 1.08 ± 0.11 |
Permeabilized BON cells were incubated with purified ALF4−-activated Gαi subunits. The effects observed by the added proteins could not be overcome after prolonged heating to 100°C, which contrasts with the effects observed after application of Gαo2 and must be attributed to solvent contaminants.