Fig. 1.
Representative PAGE gel showing specific hybridization of 32P-labeled NGF antisense riboprobe to total cellular RNA from rat dentate gyrus. Representative gel shown for NGF riboprobe hybridized against 50 μg of total cellular RNA extracted from dentate gyrus subfield dissections of naive control, platinum wire lesion, and experimental seizure (4, 10, and 24 hr after HL) rat hippocampus. After RNase digestion of hybridized samples, protected fragments were run out on a 4% polyacrylamide–8m urea gel and visualized autoradiographically to verify specific hybridization.