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SHyT quantitation of NGF mRNA levels after HL. Fifty micrograms of total cellular RNA were extracted from the dentate gyrus of naive control, platinum wire lesion, and experimental seizure (4, 10, and 24 hr after HL) rats and analyzed for levels of NGF mRNA using the SHyT. Data are the mean ± SEM of values obtained from four independent experiments. *p < 0.05 compared with naive control values; Fisher's PLSD post hoc analysis.
