Fig. 4.

Characterization of the NGFAP1 oligomer probe in EMSA experiments. a, EMSA comparison of dentate gyrus nuclear extracts from naive and 4 hr post-HL rats was performed in the presence of excess unlabeled NGFAP1 probe. Elimination of the AP-1 shifted oligomer band by the cold probe indicates the specificity of the AP-1 protein–NGFAP1 probe interaction (compare with nonspecific binding band, which is unchanged by presence of cold oligomer).b, EMSA comparison of dentate gyrus extracts from naive control, platinum wire lesion, and 4 hr post-HL rats, indicating that surgical technique and lesion placement were not sufficient to induce AP-1 binding. All experiments repeated three times with similar results.