Fig. 6.

Supershift analysis of AP-1 composition with Jun family antibodies after HL. Dentate gyrus nuclear extracts from rats 4, 10, and 24 hr after HL were incubated with antibodies against various Jun family members and then EMSA-probed with ³²P-labeled NGFAP1 oligomer. Antibodies were preincubated with antigenic blocking peptide (+ lanes) or mock peptide (−lanes) to control for nonspecific antibody effects on AP-1–NGFAP1 binding. Supershifting and/or diminution of the AP-1-shifted oligomer band by a particular antibody is indicative of participation of the respective protein in the AP-1 complex. Experiments were repeated four times with similar results.