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Supershift analysis of AP-1 composition with Fos family antibodies after HL. Dentate gyrus nuclear extracts from rats 4, 10, and 24 hr after HL were incubated with antibodies against various Fos family members and then EMSA-probed with 32P-labeled NGFAP1 oligomer. Antibodies were preincubated with antigenic blocking peptide (+ lanes) or mock peptide (−lanes) to control for nonspecific antibody effects on AP-1–NGFAP1 binding. Experiments were repeated four times with similar results.
