Fig. 2.

Effect of heterologous overexpression of Gβ2 and β5 alone or with Gγ2, Gγ3, and Gγ4 on facilitation and NE-mediated inhibition of Ca²⁺ currents.A, B, Summary graphs of mean ± SEM basal facilitation and Ca²⁺ current inhibition for neurons expressing either β2 or β5 alone and combined with several γ subunits. Final concentration of cDNA injected was 10 ng/μl per subunit. Basal facilitation and Ca²⁺ current inhibition were calculated as described in Figure1E. Note that scales for both parameters are the same. Numbers in parentheses indicate the number of experiments. Insets show superimposed current traces evoked with the double-pulse voltage protocol (illustrated in Fig. 1D) in the absence or presence of 10 μm NE for β2γ4- and β5γ2-expressing neurons. *p < 0.05 versus control; **p < 0.01 versus control.