Fig. 3.
Effect of thyroid hormone manipulation on gene expression in the GD16 fetus (Experiment II). Quantitative analysis of film autoradiograms after in situ hybridization for NSP, Oct-1, and 6C are described in Materials and Methods. Bars represent mean ± SEM of the film density (converted to % controls ± CV) over the cortex, with number of dams per group noted within each bar. All animals were killed at 9 A.M. (0900) on GD16. Groups differed in the timing of T4 injection as shown below the ordinate. Open bars, Euthyroid dams (no MMI);closed bars, hypothyroid dams (MMI).a Significantly different from hypothyroid dams receiving no injection (p < 0.05).b Significantly different from hypothyroid dams with identical timing of acute T4 treatment (p < 0.05).