Table 2.

Characteristics of plasmids used to prepare cRNA probes forin situ hybridization

cDNA	Vector	Insert length (bp)	Antisense2-a	Sense2-b	Reference
TRα1	pGEM3Z	681	EcoRI/Sp6	HindIII/T7	Bradley et al., 1989
TRβ1	pGEM3Z	681	EcoRI/Sp6	HindIII/T7	Bradley et al., 1989
2A	pCRII	461	EcoRV/Sp6	BamHI/T7	Table1
18C	pCRII	146	BamHI/T7	EcoRV/Sp6	Table1
6C	pCRII	185	EcoRV/Sp6	BamHI/T7	Table1
15A	pCRII	423	EcoRV/Sp6	BamHI/T7	Table1
4C	pCRII	212	EcoRV/Sp6	BamHI/T7	Table1
5C	pCRII	212	EcoRV/Sp6	KpnI/T7	Table1
17C	pCRII	241	BamHI/T7	EcoRV/Sp6	Table1
17CB	pBluescript	172	EcoR1/T3	ClaI/T7	Table1
18AL	pCRII	100	EcoRV/Sp6	BamHI/T7	Table1
18AS	pCRII	100	BamHI/T7	EcoRV/Sp6	Table1
14C	pBluescript	220	BamHI/T7	XhoI/T3	Table1
TRH	pSp65	1200	HindIII/Sp6		Lechan et al., 1986

^F2-a The enzyme used for linearizing the plasmid and the polymerase used to produce the complementary (antisense) RNA probe.

^F2-b The enzyme used for linearizing the plasmid and the polymerase used to produce the control (sense) RNA probe.