Table 3.
Serum levels of total T3, total T4, and TSH, and expression levels of TRH mRNA in the PVN of adult thyroid hormone-manipulated animals (Experiment IV)
| Thyroid status | T3(ng/ml) | T4 (ng/ml) | TSH (ng/ml) | TRH (% control) |
|---|---|---|---|---|
| Control | 0.790 ± 0.055 (5) | 55.750 ± 1.335 (4) | 12.013 ± 2.742 (5) | 100.00 ± 2.171 (5) |
| T3 | 6.160 ± 0.517 (6)** | UD (6) | 5.110 ± 1.371 (5) | 85.50 ± 2.185 (6)** |
| MMI + PTU | 0.590 ± 0.042 (6) | UD (6) | 29.436 ± 6.903 (6)* | 111.37 ± 1.640 (6)** |
| MMI + PTU + T3 | 6.620 ± 1.009 (6)** | UD (6) | 4.161 ± 0.828 (5) | 85.87 ± 3.535 (6)** |
Serum T3, T4, and TSH values represent mean ± SEM (n). TRH values represent mean ± SEM (n) of film density (converted to % control ± CV) over the PVN.
* Significantly different from control euthyroid rats receiving saline injections (p < 0.05).
** Significantly different from control euthyroid rats receiving saline injections (p < 0.01).
UD, Undetectable. Serum hormone levels were assigned the value of the lower assay detection limit for statistical purposes.