Fig. 8.

Photomicrographs illustrating TH immunostaining of a sagittal section containing the PFC (A, B) and the nucleus accumbens (C, D) in a control rat (A, C) and a rat 14 d after withdrawal from amphetamine treatment (B, D). In the PFC of the amphetamine-withdrawn rat (B), the levels of TH immunoreactivity are substantially similar to that found in the control rat (A). In addition, there appears to be an increase in TH staining in the cortex of amphetamine-withdrawn rats, as revealed by the presence of numerous TH-immunoreactive somata (B, D, arrows) that are less frequently observed in the control cortices. Similar levels of TH staining were also noted in the ventral striatum, as shown in an amphetamine-withdrawn rat (D) when compared to a vehicle-treated control (C). E, An example of TH-immunoperoxidase stain of DA cell bodies in VTA and SN, at bregma −5.6 mm. VS, Ventral striatum; PFC,prefrontal cortex; VTA, ventral tegmental area;SN, substantia nigra; cc, corpus callosum; bv, blood vessel. Scale bar ( inA), 200 μm.