Fig. 9.

Photomicrographs illustrating nitric oxide synthase immunoreactivity in the PFC (A, B) and the nucleus accumbens (C, D) in control rats (A, C) and in rats at 14 d after withdrawal from amphetamine (B, D). A, B, Comparisons of peroxidase-stained NOS-positive neurons in the PFC of control (A) and amphetamine-withdrawn (B) rats in a sagittal plane. Lower levels of NOS immunoreactivity were observed in the deep layer of PFC of amphetamine-withdrawn rats. C, D, Comparisons of Texas Red-labeled NOS-containing neurons in accumbens at an equivalent AP level in a coronal plane. Significantly fewer NOS-containing neurons were noted in these brain regions in amphetamine-treated rats (D) as compared to those observed in paired controls (C; Table 1). Arrows indicate NOS-positive neurons; PFC, prefrontal cortex;VS, ventral striatum; ac, anterior commissure. Scale bar (in A), 250 μm.