Fig. 2.

Localization GRK1 in rod and cone photoreceptor outer segments. Immunocytochemistry was performed as described in Materials and Methods on retinal sections from WT mice; 1–3show images made from a single section of WT retina and 4–6from a single section of GRK1 −/− retina. GRK1-specific antibody 8585 was raised in rabbit against the first 50 amino acids of bovine GRK1; its binding was visualized with fluorescein-conjugated goat anti-rabbit antibody (1, 3, 4, 6). Biotin-conjugated PNA, which binds to cone membranes, was used to localize cone photoreceptors; its binding was visualized by conjugation with Texas Red-conjugated streptavidin (2, 3, 5, 6). The outer segment layer of the retina of WT mice is strongly labeled with 8585 antibody (1); WT cones seen to be labeled with PNA in 2are also clearly labeled with 8585 (arrows in1–3). Both rod and cone outer segments of GRK1 −/− mouse are negative for GRK1 (4). Scale bar, 30 μm. Layer abbreviations are given in Figure 1.