Fig. 4.

Quantitation of BrdU-labeled nuclei using a sector template. BrdU-positive nuclei were counted using a template that divides the spinal cord into radial sectors. Dorsal, lateral, and ventral sectors from C7 are compared. The number of BrdU-positive nuclei is expressed as a percentage of the total number of DAPI-labeled nuclei for each region. Comparisons are corrected for surface area. No statistical differences were noted among sectors. This finding indicates that cell division cannot be modeled by dorsal to ventral gradients. Non-pooled sectors were also compared, but no statistical differences were found (data not shown).