Fig. 9.
A2A receptor antagonists block the action of domoate on evoked IPSCs. A, IPSCs were evoked by intrastriatal stimulation (at a rate 0.2 Hz) in the presence of NBQX (1 μm). Top traces, Domoate (500 nm for 2 min) reversibly decreased evoked IPSC amplitude.Bottom traces, In the same cell, perfusion of the slice with the selective A2A antagonist ZM 241385 (1 μm) prevented the action of domoate on evoked IPSC amplitude. The dotted line represents the level of the control inward current. B, For the same experiment, plot of the amplitude of evoked IPSCs as a function of time. Domoate is applied at the time indicated by the open horizontal bars. As indicated by the bottom horizontal bar, ZM 241385 (1 μm) is perfused several minutes before the second application of domoate. C, Histogram of the average (± SEM) inhibition by domoate (500 nm for 2 min) of evoked IPSC amplitude in control conditions (n = 18), in the presence of ZM 241385 (1 μm) (n = 7), and in the presence of SCH-58261 (1 μm) (n = 6). For both antagonists, the difference in inhibition versus domoate alone was significant withp < 0.001.