Fig. 3.

Light microscopic comparison of horizontal brain sections of fh/fh and unaffected littermates at P0, P7, P14, and P21. Halothane-anesthetized animals were intracardially perfused and then fixed and sectioned on a vibratome at 50 μm. Horizontal sections were mounted on gel-coated slides and stained with cresyl violet. The fh/fh brain is significantly smaller at all ages. Although all brain regions are reduced in size, the cerebral cortex and cerebellum are particularly affected infh/fh.