Fig. 7.

BrdU pulse labeling reveals a comparable rate of proliferation in fh/fh and unaffected cortex. Animals were injected with 60 μg/g of BrdU 1 hr before they were killed. Paraffin sections were processed for immunohistological detection of BrdU incorporation as described in Materials and Methods. BrdU labeling in the E19 unaffected (left) and fh/fh(right) cortex and P1 cerebellum is shown. The percentage of total BrdU-positive cells in the PVE of the cerebral cortex and EGL of the cerebellum has been quantified (n = 4) and shown graphically. No significant difference in the proportion of BrdU-positive cells was observed in cortical or cerebellar sections between unaffected andfh/fh animals.