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. 2000 Aug 1;20(15):5792–5801. doi: 10.1523/JNEUROSCI.20-15-05792.2000

Fig. 6.

Fig. 6.

The TCA projection is variably reduced innetrin-1 −/− mice. Coronal sections of E17.5 (A, B′) and E18.5 (C–H) mouse brains from wild-type (A, A′, C, C′, E, E′, G) andnetrin-1 −/− (B, B′, D, D′, F, F′, H) littermates, showing DiI-labeled cell bodies (G, H), axons (A′–D′, E, F), and bisbenzimide counterstain of the same sections (A–D, E′, F′). DiI was implanted into the ventroposterior thalamic nucleus (VP) to anterogradely label VP axons (A′–D′, E, F), or into somatosensory cortex to retrogradely label VP neurons (G, H). I, Quantification of retrogradely labeled VP neurons. In wild-type animals at E17.5 (A′) and E18.5 (C′), TCAs pass through the internalcapsule (ic) to reach cortex, extend intracortically within the subplate (SP;A′, B′, arrowheads), and extend branches into the cortical plate (CP). In contrast, innetrin-1 −/− littermates at E17.5 (B′) and E18.5 (D′), the TCA projection is reduced (B′, D′, arrowheads). E, F, Higher magnification images of the TCA projections shown in C′and D′, respectively. In the wild-type mouse, a substantial population of anterogradely labeled VP axons is present in the subplate, and their branches densely invade the cortical plate (E). In the netrin-1 −/− littermate, fewer labeled VP axons reach the subplate, and fewer branches extend into the cortical plate (F).G, H, The number of VP neurons retrogradely labeled by DiI injected into somatosensory cortex in netrin-1 −/− mice (H) is considerably reduced compared to their matched wild-type littermates (G).Dashed white lines mark the lateral border of the VP nucleus. I, The number of retrogradely labeled VP neurons in netrin-1 −/− mice plotted as a percentage of the number in wild-type littermates paired for injection size and location. Case #1 corresponds to the pair shown inG and H. The mean value for the five cases is also plotted. LG, Lateral geniculate nucleus;MZ, marginal zone; tr, thalamic radiations. Scale bars: A–D′, 200 μm (also applies to A–C and A′–C′); E, F′, 100 μm; G, H, 100 μm. Dorsal is to thetop, and lateral is to the right.