Table 2.

Image analysis of ubiquitin-positive particles

	Tg (+) control	Tg (+) ibuprofen	Percent change	p value
Numbers of small particles	77.05  ± 13.8	40.5  ± 10.1	↓47.5%	0.03
Area of small particles (μm2)	100.1  ± 6.8	131.1  ± 14.1	↑31%	0.03
% area of small particle	0.049  ± 0.01	0.016  ± 0.005	↓67.3%	0.005
Total area of small particles (μm2)	7865.1  ± 1321.1	3871.4  ± 876.1	↓50.8%	0.01

Using NIH Image public domain software, data were analyzed by two-way ANOVA (diet × region). Analyses were performed on 1618 small particles in Tg+ mice fed control diet (n = 7; three sections per animal) and 505 small particles in Tg+ mice fed ibuprofen diet (n = 5; three sections per animal). Values are mean ± SE. Treatment effects were not dependent on region. Square root or logarithmic transformations were required for equality of variance for total area, size, and percentage area of small particles.