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Western blot of lysates from vector-infected astrocytes. Cell lysates were treated with (+) or without (−) endoH to remove unmodified N-linked glycosylations. Five (R184Q or D598N) or 12 (all others, so lower MW band at 200 kDa would be visible) μg of protein were loaded per lane. The upper MW band, the fully glycosylated form of L1 (∼210 kDa), is resistant to endoH (compare − with + in L1, S1194L, and L3–1), whereas the lower MW band of L1 is sensitive.
