Fig. 6.
The mitochondria uncoupler 2,4-dinitrophenol (DNP) mimicked the effects of anoxia as monitored by extracellular voltage changes. a, The paradigm of Figure1a was repeated in WT Drosophila, except that an additional pipette containing either 1 or 10 mm DNP in Ringer's solution was inserted into the retina, and DNP was applied by pressure injection (during the 30 sec break in a andb). It is estimated that DNP is diluted ∼10- to 40-fold in the eye. Control injections of Ringer's solution without DNP had no effect. b, The paradigm of awas repeated in the double mutanttrpl302;trpP343.