Fig. 7.

Immunoblot showing the effects of ODN treatment on KOR protein levels in rat DRG. Tissue extracts were prepared from L4, L5, L6, and S1 DRG of saline (C), antisense ODN-treated (AS), and mismatch ODN-treated (MM) rats. Samples were normalized for protein content using BCA quantification. Each lane was loaded with ∼100 μg of protein, and immunoreactive bands were revealed at 43 and 70 kDa with an antibody directed against an internal region of the cloned rat KOR. Antisense ODN, but not mismatch ODN treatment, caused a clear downregulation of KOR protein at all the DRGs examined. Similar results were obtained from duplicate experiments.