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Vti1a-β was not found in the exocytic SNARE complex. SNARE complexes were isolated from Triton X-100 extracts of synaptosomes using antisera against Vti1a, SNAP-25, syntaxin 1 (Syx1), or synaptobrevin (Syb). Vti1a antiserum did not coimmunoprecipitate syntaxin 1, SNAP-25, or synaptobrevin. SNAP-25, syntaxin 1, and synaptobrevin coimmunoprecipitated as a SNARE complex.
