Fig. 2.

RA- and laminin-induced cdk5 activity and NF-H KSP tail domain phosphorylation in SH-SY5Y human neuroblastoma cells.A, Cells were treated with RA (10 μm) or without for 7 d and then grown on poly-l-lysine or laminin for 24 hr. Cell extracts were immunoprecipitated with cdk5 antibody, and the immunoprecipitates were assayed for their ability to phosphorylate histone H1. B, Equal amounts of protein from cell lysates were used in each case for Western blot analysis using anti-phospho-dependent NF-H tail domain antibody SMI31 and anti-phospho-independent NF-H antibody SMI33. PL, Poly-l-lysine; LN, laminin.