Fig. 5.

Evaluation of neurite outgrowth of human SH-SY5Y neuroblastoma cells in the presence and absence of α₁β₁ antibodies or BL-1. Cells were treated with or without RA as described in Materials and Methods and then detached with EGTA and plated on coverslips coated with 10 μg/ml laminin or 100 μg/ml poly-l-lysine in medium without serum in the presence and absence of α₁ (FB12) and β₁ (DE9) antibodies or BL-1 for 24 hr. After fixation and staining with crystal violet, five randomly selected fields were photographed in each sample. Processes were measured in 120 cells per sample, and only those longer than 15 μm were scored.PL, Poly- l-lysine; LN, laminin. α1Abs, anti-integrin α₁antibody; β1Abs, anti-integrin β₁antibody.