Fig. 3.

Western blot analysis of detergent-solubilized hippocampal slices (C; lanes 1, 2) incubated in sample buffer at 100°C (B) or 30°C (−) or cross-linked with 4% paraformaldehyde (X; lanes 3, 4) before detergent solubilization (tube slices). Forty microliters of each hippocampal homogenate were loaded on a 10% polyacrylamide gel and fractionated by PAGE. The membranes were probed with a monoclonal antibody against SNAP25 (SMI 81; Sternberger Monoclonals). The SNAP25-containing SDS-resistant complexes and the cross-linked SNAP25 complexes are compared. * indicates a SNAP25-immunoreactive band that is seen in the absence as well as the presence of cross-linking agent. The results are representative of six independent experiments.