Fig. 4.

Western blot analysis of detergent-solubilized hippocampal slices (−) and hippocampal slices cross-linked with 4% paraformaldehyde (X) before detergent solubilization (tube slices). Hippocampal slices were incubated in sample buffer at 100°C or 37°C. Forty microliters of each hippocampal homogenate were loaded on a 12% (A) or 10% (B) polyacrylamide gel and fractionated by PAGE. A, The membranes were probed with a VAMP polyclonal antisera. B, The membranes were probed with a synaptophysin monoclonal antibody (Sigma). The 55 kDa VAMP-containing protein complex comigrates with a 55 kDa synaptophysin protein complex. The top VAMP- and synaptophysin-containingbands represent the interface between the stacking and resolving gels. The results are representative of six independent experiments.