Fig. 5.

Effect of SRIF stimulation on the subcellular distribution of SST_2A receptors in dendrites of the claustrum and basolateral amygdala. Slices were incubated with or without [d-Trp⁸]-SRIF (10 nm–10 μm) in Ca²⁺-free or Ca²⁺-supplemented Ringer's. Dendrite-associated immunogold particles were counted and classified as either membrane-associated or intracellular; results are expressed as membrane-associated/total. In the absence of agonist, ∼60% of gold particles were associated with dendritic plasma membranes in both cerebral regions, irrespective of the Ca²⁺concentration in the medium. Addition of agonist induced a decrease in the percentage of receptors associated with plasma membranes, again both in the presence and absence of Ca²⁺. The agonist-induced decrease in surface receptors was prevented by adding 10 μm PAO to the incubation medium. Values are the mean ± SEM from three animals; **p < 0.01.