Fig. 6.

α-Synuclein increases iron-dependent toxicity.A, B, BE-M17 cells overexpressing wild-type, A53T, or A30P α-synuclein were treated with varying doses of FeCl₂ for 48 hr, and the viability was determined using the MTT assay (A) or the LDH assay (B). C, BE-M17 cells overexpressing wild-type, A53T, or A30P α-synuclein were treated with varying doses of H₂O₂ for 48 hr, and the viability was determined using the MTT assay. *p < 0.001, +p < 0.01 by ANOVA analysis.D, Iron stain of BE-M17 cells. a, Untransfected cells, basal conditions; b, A53T α-synuclein-expressing cells, basal conditions; c, untransfected cells, FeCl₂–H₂O₂; d, A53T α-synuclein-expressing cells, FeCl₂–H₂O₂. Under basal conditions, none of the cells showed staining for iron, but after treatment with 10 mm FeCl₂ and 100 μm H₂O₂ for 48 hr, the A53T cells showed much more iron reactivity (blue, iron stain;pink, nuclear–cytoplasmic counterstain).